MStem Cell Lab Collaborative Projects
1. Villa-Diaz LG, Pacut C, Slawny NA, Ding J, O’Shea KS, Smith GD. Analysis of the factors that limit the ability of feeder cells to maintain the undifferentiated state of human embryonic stem cells. Stem Cells Dev(2009) 18:641-651. PMID: 18764735.
2. Villa-Diaz, LG, Torisawa Y, Uchida T, Ding J, Nogueira-de-Souza NC, O’Shea KS, Takayama S, Smith GD. Microfluidic culture of single human embryonic stem cell colonies. Lab Chip(2009) 9:1749 -1755. PMID: 19495459.
3. Villa-Diaz LG, Nandivada H, Ding J, Nogueira-de-Souza NC, Kresbach PH, O’Shea KS, Lahann J, Smith GD. Synthetic polymer coatings for long-term growth of human embryonic stem cells. Nature Biotechnol (2010) 28:581-583. PMID: 20512122.
4. Smith GD, Swain JE, and Bormann, CL. Microfluidics for gametes, embryos, and embryonic stem cells. Semin Reprod Med(2011) 29:5-14. PMID: 21207330.
5. Himabindu Nandivada H, Villa-Diaz LG, O’Shea SK, Smith GD, Krebsbach PH, Lahann J. Fabrication of synthetic polymer coatings and their use in feeder-free culture of human embryonic stem cells. Nature Protoc (2011) 6:1037-1043. PMID: 21720316.
6. Rocha AM, Guerrero-Serna G, Helms A, Luzod C, Mironov S, Russell M, Jalife J, Day SM, Smith GD*, Herron TJ* (*corresponding authors). Deficient cMyBP-C protein expression during cardiomyocyte differentiation underlies human hypertrophic cardiomyopathy cellular phenotypes in disease specific human ES cell derived cardiomyocytes. J Mol Cellular Cardiology (2016) 99:197-206.
7. Smith GD, Keller L, Dorfmann A, Tran K, Stern H, Mariani B. ALS/FTD chromosome 9 open reading frame 72 expansion (C9orf72-exp) preimplantation genetic diagnosis (PGD) and disease-specific human embryonic stem cell (ds-hESC) derivation: a family’s win/win and contribution to science. Amer Soc Reprod Med 2016 (abstract).
8. Haenfler JM, Skariah G, Rodriguez CM, da Rocha AM, Parent JM, Smith GD, Todd PK. Targeted reactivation of FMR1 transcription in fragile X syndrome embryonic stem cells. Front Mol Neurosci (2018) 11: 282.
9. Moore LR, Keller L, Bushart DD, Delatorre R, Li D, McLoughlin HS, Costa MDC, Shakkottai VG, Smith GD, Paulson H. Antisense oligonucleotide therapy rescues aggresome formation in a novel spinocerebellar ataxia type 3 human embryonic stem cell line. Stem Cell Res(2019) 39:1-13.
10. Khoa LTP, Tsan YC, Mao F, Kremer D, Sajjakulnukit P, Tong X, Bhanu NV, Garcia BA, Yin L, Smith GD, Saunders TL, Bielas S, Lyssiotis CA and Dou Y. Histone acetyltransferase MOF regulates fatty acid oxidation and cellular metabolism in cell fate determination. Cell Stem Cell(2020) 27: 1-18.
11. Shahbazi MN, Wang T, Tao X, Weathersbee BAT, Sun L, Zhan Y, Keller L, Smith GD, Pellicer A, Scott RT, Seli E, Zernicka-Goetz M. Developmental potential of aneuploid human embryos cultured beyond implantation. Nature Comm(2020) 11-3987: 1-15.
12. Cloutier M, Kumar S, Buttigieg E, Keller L, Erliandri I, Lee B, Williams A, Mojica-Perez S, Monteiro Da Rocha A, Smith GD, and Kalantry S. Preventing erosion of X-chromosome inactivation in human embryonic stem cells. Nature Comm (2022), 13:2516, PMID: 35523820.
13. Ren Y, Yan Z, Yang M, Keller L, Zhu X, Lian X, Liu Q, Li R, Zhai F, Nie Y, Yan L*, Smith GD*, Qiao J* (*Co-corresponding author). Regional and developmental characteristics of human embryo mosaicism revealed by single cell sequencing. PLOS Genetics (2022) – accepted.
14. Moore RL, Keller L, Paulson HL*, Smith GD* (*Co-corresponding author). Generation of Spinocerebellar Ataxia type 3 human embryonic stem cell line UM134-1. Stem Cell Res. (2022) – accepted.
15. Erliandri I, Sagortra A, Lieberman A,* Smith GD* (*Co-corresponding author). Generation of Spinal/Bulbar Muscular Atrophy (Kennedy Disease) disease-specific human embryonic stem cell line UM197-1. Stem Cell Res. (2022) – in progress.
16. Smith GD, Erliandri I, Keller L, Johnson TRB. Generation and characterization of sibling and non-sibling C9orf72-expansion disease-specific human embryonic stem cell for the study of frontotemporal dementia (FTD) and amyotrophic lateral sclerosis (ALS). Stem Cell Res. (2022) – in progress.
Known Publications using MStem Cell Lab Derived UM-hESCs
1. Chen HM, DeLong CJ, Bame M, Rajapakse I, Herron TJ, McGinnis MG, O’Shea KS. Transcripts involved in calcium signaling and telencephalic neuronal fate are altered in induced pluripotent stem cells from bipolar disorder patients. Nature Transl Psychiatry (2014).
2. Gaitas A, Malhotra R, Li T, Herron T, Jalife J. A device for rapid and quantitative measurements of cardiac myocyte contractility. Rev Sci Instruments (2015)
3. Taniguchi K, Shao Y, Townshend RF, Tsai YH, DeLong CJ, Lopez SA, Gayen S, Freddo AM, Chue DJ, Thomas DJ, Spence JR, Margolis D, Kalantry S, Fu J, O’Shea KS, Gumucio DL. Lumen formation is an intrinsic property of isolated human pluripotent stem cells. Stem Cell Report (2015).
4. Dye BR, Hill DR, Ferguson MAH, Tsai YH, Nagy MS, Dyal R, Wells JM, Mayhew CN, Nattiv R, Klein OD, White ES, Deutsch GH, Spence JR. In vitro generation of human pluripotent stem cell derived lung organoids. eLIFE(2015).
5. Ilic, D and Ogilvie C. Concise review: human embryonic stem cells-what have we done? what are we doing? where are we going? Stem Cell (2016).
6. Herron TJ, Rocha AM, Campbell KF, Ponce-Balbuena D, Willis C, Guerrero-Serna G, Liu Q, Klos M, Musa H, Zarzoso M, Bizy A, Furness J, Anumonwo J, Mironov S, Jalife J. Extracellular matrix-mediated maturation of human pluripotent stem cell-derived cardiac monolayer structure and electrophysiological function. Circ Arrhythm Electrophysiol (2016).
7. Shao Y, Taniguchi K, Gurdziel K, Townshend RF, Xue X, Yong KMA, Sang J, Spence JR, Gumucio DL, Fu J. Self-organized amniogenesis by human pluripotent stem cells in a biomimetic implantation-like niche. Nature Materials (2017).
8. Cruz-Acuna R, Quiros M, Farkas AE, Dedhia PH, Huang S, Siuda D, Garcia-Hernandez V, Miller AJ, Spence JR, Nusrat A, Garcia AJ. Synthetic hydrogels for human intestinal organoid generation and colonic wound repair. Nature Cell Biol(2017).
9. Dye RB, Dedhia PH, Miller AJ, Nagy MS, White ES, Shea LD, Spence JR. A bioengineered niche promotes in vivo engraftment and maturation of pluripotent stem cell derived human lung organoids. eLIFE(2017).
10. Merkle FT, Ghosh S, Kamitaki N, Mitchell J, Avior Y, Mello C, Kashin S, Mekhoubad S, Ilic D, Charlton M, Saphier G, Handsaker RE, Genovese G, Bar S, Benvenisty N, McCarrol SA, Eggan K. Human pluripotent stem cells recurrently acquire and expand dominant negative p53 mutations. Nature (2017).
11. Torres Collazo BJ and Cebrian-Ligero C. Developing renal organoids using human embryonic stem cells. FASEB (2017).
12. Shao Y, Taniguchi K, Townshend RF, Miki T, Gumucio DL, Fu J. A pluripotent stem cell-based model for post-implantation human amniotic sac development. Nature Comm (2017).
13. Miller AJ, Hill DR, Nagy MS, Aoki Y, Dye BR, Chin AM, Huang S, Zhu F, White ES, Lama V, Spence JR. In vitro induction of in vivo engraftment of lung bud tip progenitor cells derived from human pluripotent stem cells. Stem Cell Reports (2018).
14. Miller AJ, Dye BR, Ferrer-Torres D, Hill DR,Overeem AW, Shea LD, Spence JR. Generation of lung organoids from human pluripotent stem cells in vitro. Nat Prot (2019).
15. Ashraf NS, Sutton JR, Yang Y, Ranxhi B, Libohova K, Shaw ED, Barget AJ, Todi SV, Paulson HL, Costa MC. Druggable genome screen identifies new regulators of the abundance and toxicity of ATXN3, the Spinocerebellar Ataxia Type 3 disease protein. NeuroBio Dis (2020). 16. Merkle FT, Ghosh S, Genovese G, Handsaker RE, Kashin S, Meyer D, Karczewski KJ, O’Dushlaine C, Pato C, Pato M, MacArthur DG, McCarroll SA, Eggan K. Whole-genome analysis of human embryonic stem cells enables rational line selection based on genetic variation. Cell Stem Cell(2022).